Giera M. Clinical Metabolomics. Methods and Protocols 2ed 2024

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Giera M. Clinical Metabolomics. Methods and Protocols 2ed 2024

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Textbook in PDF format This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. Chapters explore the core of several promising initiatives evolving around personalized health care and precision medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Metabolomics: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. Preface Contributors Clinical Metabolomics (Pre)Clinical Metabolomics Analysis LC-MS-Based Metabolomics Global Metabolomics Using LC-MS for Clinical Applications Modular, Scalable, and Customizable LC-HRMS for Exposomics Distinguishing Artifactual Fatty Acid Dimers from Fatty Acid Esters of Hydroxy Fatty Acids in Untargeted LC-MS Pipe... Advances in Chiral Metabolomic Profiling and Biomarker Discovery Stable Isotope Tracing Experiments Using LC-MS Quantification of Total Ketone Bodies in Biological Matrices Using Stable Isotope-Labeled Internal Standards and RP... LC-MS/MS Quantification of Endocannabinoids in Tissues Prostaglandin Metabolites Analysis in Urine by LC-MS/MS A Method for Analysis of Oxidized Phospholipids from Biological Samples Using Mass Spectrometry Nonenzymatic Oxidized Polyunsaturated Fatty Acid Products in Human Plasma and Urine Samples by LC-QTOF-MS/MS Reversed-Phase Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Method for High-Throughput Lipidomic... High-Throughput RPLC-MS/MS Quantification of Short- and Medium-Chain Fatty Acids Long Chain Base Profiling with Multiple Reaction Monitoring Mass Spectrometry Sphingolipid Analysis in Clinical Research Recommendations for Accurate Lipid Annotation and Semi-absolute Quantification from LC-MS/MS Datasets GC-MS-Based Metabolomics Rapid Analysis of Sterols in Blood-Derived Samples SFC-MS-Based Metabolomics Ultrahigh-Performance Supercritical Fluid Chromatography-Mass Spectrometry in the Clinical Lipidomic Analysis Quantification of Octadecanoids in Human Plasma Using Chiral Supercritical Fluid Chromatography-Tandem Mass Spectr... Rapid Lipid Mediator Profiling by Convergence Chromatography-MS/MS FIA-MS-Based Metabolomics Analysis of the Mammalian Lipidome by DMS Shotgun Lipidomics Differential Mobility Spectrometry-Based Cardiolipin Analysis CE-MS-Based Metabolomics CE-MS-Based Clinical Metabolomics of Human Plasma NMR-Based Metabolomics Quantitative Blood Serum IVDr NMR Spectroscopy in Clinical Metabolomics of Cancer, Neurodegeneration, and Internal... Lipoprotein (Sub)Fraction Analysis on the Bruker B.I. LISA Platform NMR-Based Stable Isotope Tracing of Cancer Metabolism NMR-Based Analysis of Cellular Central Energy Metabolism and Exchange Rates MALDI-MS-Based Metabolomics In Situ Isotope Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging Data Processing, Analysis, and Bioinformatics Extracting Knowledge from MS Clinical Metabolomic Data: Processing and Analysis Strategies Application of a Computational Metabolomics Workflow for the Diagnosis of Inborn Errors of Metabolism in a Laborat... Index