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Wery M. mRNA Decay. Methods and Protocols 2025

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Wery M. mRNA Decay. Methods and Protocols 2025

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Category: Other
Total size: 17.35 MB
Added: 2025-03-10 23:39:03

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Info Hash: 4BD4B04CBB3E6C15827D491D41E06F02CF4880A2
Last updated: 4.2 hours ago

Description:

Textbook in PDF format This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls