Kroemer G. Liver Carcinogenesis. Methods and Protocols 2024

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Kroemer G. Liver Carcinogenesis. Methods and Protocols 2024

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Textbook in PDF format This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction of ectopic implantation. The second half of this book covers a diverse array of techniques applied to characterize the biochemical and cellular composition of hepatic malignancies that operate at the single-cell and histological levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Liver Carcinogenesis: Methods and Protocols, is a valuable resource for students and scientists who are interested in driving progress in the important field of liver cancer research. Orthotopic Model of Hepatocellular Carcinoma in Mice Diethylnitrosami​ne Induction of Hepatocarcinogen​esis in Mice Diethylnitrosami​ne-Induced Liver Tumorigenesis in Mice Under High-Hat High-Sucrose Diet:​ Stepwise High-Resolution Ultrasound Imaging and Histopathologica​l Correlations A Mouse Model of Non-Alcoholic Steatohepatitis and Hepatocellular Carcinoma Induced by Western Diet and Carbon Tetrachloride A Mouse Model of Hepatocellular Carcinoma Induced by Streptozotocin and High-Fat Diet Hydrodynamic Transfection of Hepatocytes for the Study of Hepatocellular Carcinogenesis Experimental Model of Biliary Tract Cancers:​ Subcutaneous Xenograft of Human Cell Lines in Immunodeficient Nude Mice Oncogene-Driven Induction of Orthotopic Cholangiocarcino​ma in Mice Isolation of Primary Mouse Hepatocytes and Non-Parenchymal Cells from a Liver with Precancerous Lesions Flow Cytometry Assessment of Lymphocyte Populations Infiltrating Liver Tumors Immunofluorescen​t Staining of Human Hepatic Multicellular Spheroids:​ A Model for Studying Liver Diseases Single-Cell Characterization​ of the Tumor Ecosystem in Liver Cancer Chromatin and DNA Dynamics in Mouse Models of Liver Cancers Targeted Analysis of Glycerophospholi​pids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer Biomarker Identification in Liver Cancers Using Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) Imaging:​ An Approach for Spatially Resolved Metabolomics Kinetic Modeling of Hepatic Metabolism and Simulation of Treatment Effects