Rigler R. Fluorescence Correlation Spectroscopy. Theory and Applications 2001

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Rigler R. Fluorescence Correlation Spectroscopy. Theory and Applications 2001

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Textbook in PDF format Fluorescence correlation spectroscopy (FCS) was developed in order to char­ acterize the dynamics of molecular processes in systems in thermodynamic equilibrium. FCS determines transport and chemical reaction rates from mea­ surements of spontaneous microscopic thermally driven molecular concentra­ tion fluctuations. Since its inception, and particularly in recent years, techni­ cal and conceptual advances have extended the range of practical applicability and the information obtainable from FCS measurements. Improvements in microscopy, data acquisition, and data processing have greatly shortened the time required for FCS measurements. FCS can now be routinely applied to labile systems such as cells, and for the acquisition of large volumes of data as required for high-throughput screening. Cross correlation methods pro­ vide a powerful tool for characterizing interactions among different molecular species. Analysis of the amplitude of concentration fluctuations can provide a wealth of information about aggregation/polymerization process and the compositions of mixtures. Furthermore, FCS provides a bridge between conventional measurements of dynamic processes on a macroscopic concentration scale and the currently developing field of single molecule measurements. Both FCS and single mole­ cule approaches measure directly stochastic fluctuations in molecular pro­ perties, and so must be analyzed by statistical methods to yield conventional phenomenological parameters. As commonly practiced, FCS yields these phe­ nomenological parameters, e. g. , diffusion coefficients and chemical rate con­ stants, directly in terms of a fluorescence fluctuation autocorrelation func­tion. Introduction Introduction FCS in the Analysis of Molecular Interactions Fluorescence Correlation Spectroscopy of Flavins and Flavoproteins Fluorescence Correlation Spectroscopy in Nucleic Acid Analysis Strain-Dependent Fluorescence Correlation Spectroscopy: Proposing a New Measurement for Conformational Fluctuations of Biological Macromolecules Applications of FCS to Protein-Ligand Interactions: Comparison with Fluorescence Polarization FCS at the Cellular Level FCS-Analysis of Ligand-Receptor Interactions in Living Cells Fluorescence Correlation Microscopy (FCM): Fluorescence Correlation Spectroscopy (FCS) in Cell Biology FCS and Spatial Correlations on Biological Surfaces Applications in Biotechnology, Drug Screening, and Diagnostics Dual-Color Confocal Fluorescence Spectroscopy and its Application in Biotechnology Nanoparticle Immunoassays: A new Method for Use in Molecular Diagnostics and High Throughput Pharmaceutical Screening based on Fluorescence Correlation Spectroscopy Protein Aggregation Associated with alzhiemer and Prion Diseases Environmental Analysis and Monitoring Application of FCS to the Study of Environmental Systems Photophysical Aspects of FCS Measurements Environmental Analysis and Monitoring Fluorescence Correlation Spectroscopy: Genesis, Evolution, Maturation and Prognosis ConfoCor 2 — The Second Generation of Fluorescence Correlation Microscopes Antibunching and Rotational Diffusion in FCS Cross-correlation analysis in FCS Cross-correlated Flow Analysis in Microstructures Introduction to the Theory of Fluorescence Intensity Distribution Analysis Photon Counting Histogram Statistics High Order Autocorrelation in Fluorescence Correlation Spectroscopy FCS in Single Molecule Analysis